 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Arginine Enhancement of Cell Dissociation in Suspension-Cultured of Aromatic Rice Cells (Oryza sativa L. var Khao Dawk Mali 105)) ผู้เขียน: ดร.นิตย์ศรี แสงเดือน, ศาสตราจารย์เกียรติคุณ ,  Pranot Klamsomboon สื่อสิ่งพิมพ์:pdf AbstractSuspension culture of Oryza sativa L. var. Khao Dawk Mali 105 was initiated from embryogenic region of callus derived from mature seeds cultured in the modified N6 medium supplemented with 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 0.25 mg/l kinetin, 2 % sucrose, 5 mM proline and various nitrogen sources. Three types of suspension-cultured medium (SM) with the supplementation of inorganic nitrogen (SM1), or organic nitrogen plus arginine (SM2), or inorganic nitrogen plus arginine (SM3) were used.The suspension-cultured of rice callus in the SM gave cytoplasmically dense cell and homogeneous aggregation cell size larger than 1 mm, while those in the SM2 produced cytoplasmically clear cell and aggregated cell size smaller than 1 mm. The addition of arginine to SM with inorganic nitrogen (SM3) seemed to combine the desirable characters of finely dispersed cell aggregate and cytoplasmically dense cell. It was revealed by scanning electron micrographs indicating a difference in intercellular layer while cultured in 1 mM arginine containing SM medium was less deeply embedded in intercellular layer than in arginine-free medium. A finely dispersed cell suspension was obtained from rice callus tissue in SM supplemented with 1mM arginine. These results suggested that the suspension-cultured medium with inorganic nitrogen plus arginine should be suitable for generating an established suspension cell line for selection experiment as well as protoplast isolation. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histological and Scanning Electron Observations on Embryogenic and Non-embryogenic Calli of Aromatic Thai Rice (Oryza sativa L. cv. Khao Daw Mali 105)) ผู้เขียน:ดร.นิตย์ศรี แสงเดือน, ศาสตราจารย์เกียรติคุณ, Pranot Klamsomboon สื่อสิ่งพิมพ์:pdf AbstractTwo types of callus proliferation namely, embryogenic (E) and non-embryogenic (NE) calli were obtained by culturing mature rice (Oryza sativa L. cv. Khao Daw Mali 105) seeds in modified MS medium supplemented with 2 mg/l 2,4-D, 10 mM proline and 3% sucrose. Both light and scanning electron micrographs were employed to distinguish the surface details as well as cell shape of E and NE calli. Light micrographs of E callus was compact, nodular, knobs, white or creamy. NE callus was soft, friable, unorganized light-stained cells, translucent, watery and light yellow. Scanning electron micrographs indicated distinct morphology of cells shape. NE cell shape was long-like tubular and loosely arranged cells. E callus comprised nodular or knobby, quite deep embedded and tightly packed cell was similar to typical E callus. Histological observations of 5 ?m of callus paraffin sections revealed that E callus occurred on the surface as well as in the deeper regions of the callus. The abundance of vessel tracts in the callus which was consisted of a high content of vessel elements, rounded and cytoplasmically dense cells with totipotency. This may imply that nutrients are easily transported via these vessel tracts for the nourishment of growing somatic embryos or leafy structure. The number of vessel elements appeared to be a crucial factor to determine the E and NE calli development. |